Department of Physics and Astronomy
Clemson University, Kinard Lab of Physics, G01, Lecture Hall
Thursday, September 13, 4:00 pm
Multiparameter Fluorescence Spectroscopy for Structural Dynamics of biomolecules
Abstract: Single molecule Förster Resonance Energy Transfer experiments are rich in information of biomolecules. By time-stamping photon detection events with high temporal resolution, it is possible to construct various data representations that highlight the time evolution of multiple fluorescence parameters reflecting the structure and dynamics of molecules of interest. This can include the FRET efficiency, the fluorescence anisotropy, fluorescence decay, or the correlation of fluorescence fluctuations. Treating each representation independently provides only limited information. Nonetheless, the holistic nature of multiparameter fluorescence spectroscopy (MFS) allows for joint analysis of single molecules, thus allowing correction for potential artifacts, reducing uncertainty, and linking the structure and dynamics to the functions of biomolecules. Several examples of recent work are presented, highlighting the ample capability of MFS.
Bio: Dr. Hugo Sanabria joined the department of Physics and Astronomy in January 2014. He graduated from Tec of Monterrey, Mexico with a B.S. in Physics Engineering. He pursued a M.S. and Ph.D. in Physics at the University of Houston under the supervision of Dr. John H. Miller. His work focused on application of dielectric spectroscopy to study the electrical response of polyelectrolytes, in particular, the study of cytoskeletal filaments like microtubules. Dr. Sanabria was awarded an NIH training fellowship to follow his postdoctoral research with Dr. M. Neal Waxham at University of Texas Health Science Center in Houston. There he studied Ca2+ signaling proteins involved in learning and memory processes at a single molecule level. Later, he was awarded an Alexander von Humboldt Fellowship at Heinrich Heine University, in Dusseldorf, Germany under supervision of Dr. Claus A. M. Seidel, where he developed and enhanced single molecule methodologies to study structure and dynamics of proteins; technology he brought to Clemson University. In 2016, he was named CU School of Health Research Faculty Scholar. The experimental set up used to study single molecules is one of a kind in the United States, pioneering structural dynamics of biomolecules using fluorescence methodologies. More recently, Dr. Sanabria received the prestigious NSF Career 2018 award. His research is funded through NSF and NIH.
Refreshments will be served after the presentation in the P&A Café.
Thursday, September 13 at 4:00pm to 5:00pm
Kinard Laboratory of Physics, G01 Kinard Lab
140 Delta Epsilon Ct., Clemson, SC 29634, USA